Knowing how to calculate dilutions is crucial to the safe practice of aromatherapy.

Serial Dilution Steps
Series Dilution Table
How To Serial Dilution

It’s not enough to simply say “dilute before use on the skin”—this is insufficient information. You need to know which dilutions are safe and how to calculate them. With that information, you can use a chart like this to guide accurate dilution.

Here are a few key specific oils that have special dilution needs.

Serial Dilution Steps

To avoid the risk of skin allergy:

Dilution Table Mixing chart. This page may be printed by our customers for their use Dilution Chart. Dilution Ration 1 to 4 1 to 5 1 to 10 1 to 12 1 to 15 1 to 20 1 to 32 1 to 40 1 to 50 1 to 60 1 to 64 1 to 100 1 to 128 1 to 256: Oz. Per Gal 32 26 12 10 8 6 4 3 2 1/2 2 2 1 1 1/2. To Determine: Cost To Use. BACTERIAL DILUTIONS and A FOOL-PROOF WAY TO FIGURE THEM OUT Look at the dilution scheme below: Most questions you will be asked to answer about serial dilutions are of two types: The FIRST TYPE gives you the number of bacterial colonies found on a plate and asks for the number of bacteria per ml in the original culture. The dilutions cover the range from 1/2 to 1/100 unevenly. In fact, the 1/2 vs. 1/3 dilutions differ by only 1.5-fold in concentration, while the 1/10 vs. 1/100 dilutions differ by ten-fold. If you are going to measure results for four dilutions, it is a waste of time and materials to make two of them almost the same. Multiple Dilutions Multiple dilutions are required to decrease the sample concentration by multiple logs. If the concentration is 35,000 CFU/ml (104), and 35 CFU/ml is the target concentration, the following serial dilutions can be performed. Initial sample 3.5 x 104 CFU/ml (35,000 CFU/ml) 1 ml 9 ml 3.5 x 103 CFU/ml (3,500 CFU/ml) 9 ml 3.5 x. Table of Contents. How to Do a Viable Plate Count; 1: Why we need to dilute; 2: Setting the stage: Diluting Cold Press Coffee; 3: The dilution factor; 4: Overall dilution; 5: Design a dilution! 6: How to scale back up; 7: Count a plate! 8: Why plate more than one dilution? 9: Picking the plate count; Section 2. Practice Problems; 10.

Clove bud oil should be used at no more than 0.5%
Holy Basil oil can be used at up to 1%

To avoid the risk of phototoxicity:

Series Dilution Table

Lemon oil should be used at no more than 2%
Grapefruit oil can be used at up to 4%

Essential Calculation Class

How To Serial Dilution

You love essential oils, and you got hooked into aromatherapy – but nobody told you there will be math. Join Hana Tisserand as she guides you through calculations and conversions with ease, giving you simple tools and making sure you get it, so you will never again have to ask: “How many drops is that?”

Serial Dilution Table